Structural Evolution of Environmentally Responsive Cationic Liposome–DNA Complexes with a Reducible Lipid Linker
نویسندگان
چکیده
منابع مشابه
Structural characterization of cationic lipid–tRNA complexes
Despite considerable interest and investigations on cationic lipid-DNA complexes, reports on lipid-RNA interaction are very limited. In contrast to lipid-DNA complexes where lipid binding induces partial B to A and B to C conformational changes, lipid-tRNA complexation preserves tRNA folded state. This study is the first attempt to investigate the binding of cationic lipid with transfer RNA and...
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Cationic lipid-DNA complexes, often referred to as lipoplexes, are formed spontaneously in aqueous solutions upon mixing DNA and liposomes composed of cationic and nonionic lipids. Understanding the mechanisms underlying lipoplex formation, structure and phase behavior is crucial for their further development and design as non-viral transfection vectors in gene therapy. From a physical point of...
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The three-dimensional structure of gene products continues to be a missing lynchpin between linear genome sequences and our understanding of the normal and abnormal function of proteins and pathways. Enhanced activity in this area is likely to lead to better understanding of how discrete changes in molecular patterns and conformation underlie functional changes in protein complexes and, with it...
متن کاملStructural Studies of DNA-Cationic Lipid Complexes Confined in Lithographically Patterned MicroChannel Arrays
We have used lithographically patterned microchannel arrays with channel widths ranging from 1 to 20 um, fabricated using electron beam lithography and reactive ion etching, in structural studies of DNA-cationic lipid complexes in confinement. Various techniques have been developed for loading these DNAmembrane complexes into the microchannels or to form the complexes in situ by sequentially de...
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ژورنال
عنوان ژورنال: Langmuir
سال: 2012
ISSN: 0743-7463,1520-5827
DOI: 10.1021/la301181b